P-91: The Effects of Mesenchymal StemCell- Conditioned Medium on The Fertilization Rate and Embryo Development inNMRI Mice

Background: Normal growth of oocytes, embryos and also successful implantation depend on environmental factors such as the secretion of cumulus cells and composition of follicular and tubular fluids. Therefore, addition of serum, conditioned medium (CM) to the culture medium and also co-culture with somatic cells improve mammalian embryo development. So, in the respect to the secretion of different growth factors and cytokines by mesenchymal stem cells (MSc), the present study shows the effects of MSc-derived CM on fertilization rate and early embryo development. Materials and Methods: 60 female and 10 male NMRI mice were used to obtain the MII oocytes and capacitated sperm for IVF in the following groups: The basic fertilization medium (BFM) in control group contained T6+BSA 15mg/ml, for treated groups 1-3 was added respectively 25,50 and75% of MSc-derived CM to BFM. The fertilized oocytes transferred to embryo developmental medium (T6+BSA 4mg/ml) for 72-hour. All data was analyzed by ANOVA in SPSS 16.0 software. Results: The study showed statically a difference on fertilization rate in the treated group 3 in comparison with other ones. The results didn't show any difference on embryo developmental rate. Conclusion: It concluded that adding MSc-derived CM (50%v/v) may improve early embryo cell division.